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Polyclonal Antibody to Guanylate Binding Protein 4 (GBP4)

Image (I)

DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human GBP4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant GBP4, Human.
Knockout Varification:
Lane 1: Wild-type A431 cell lysate;
Lane 2: GBP4 knockout A431 cell lysate;
Predicted MW: 73kDa
Observed MW: 73kDa
Primary Ab: 1µg/ml Rabbit Anti-Human GBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: A431 cell lysate; Lane2: PANC1 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human GBP4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: A431 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human GBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: HepG2 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human GBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Human A431 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human GBP4 Polyclonal Antibody (Catalog PAB772Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Guanylate-Binding-Protein-4-(GBP4)-PAB772Hu01.pdf

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KO-Validated

Polyclonal Antibody to Guanylate Binding Protein 4 (GBP4)

GTP-binding protein 4; Guanine nucleotide-binding protein 4

Product No.PAB772Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB772Hu01-Recombinant Guanylate Binding Protein 4 (GBP4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GBP4. It has been selected for its ability to recognize GBP4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/m;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB772Hu01	Recombinant Guanylate Binding Protein 4 (GBP4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB772Hu01	Polyclonal Antibody to Guanylate Binding Protein 4 (GBP4)	WB; IHC; FCM