Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Mouse Heart Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse VCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse VCL Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse VCL Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant VCL, Mouse.
Western Blot; Sample:Human Lung lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Vinculin-(VCL)-PAB839Mu01.pdf

Certificate

Polyclonal Antibody to Vinculin (VCL)

MVCL; Metavinculin

Product No.PAB839Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB839Mu01-Recombinant Vinculin (VCL)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreHomo sapiens (Human), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 110 258 US$ 258 US$ 368 US$ 920 US$ 3680
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
PBMCs S-NO proteomic signature in heart failure	S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure journals:1384523
J Cell Sci	PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals Pubmed:35044463

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPB839Mu01	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB839Mu02	Recombinant Vinculin (VCL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB839Mu02	Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
PAB839Mu01	Polyclonal Antibody to Vinculin (VCL)	WB; IHC; ICC; IP.
SEB839Mu	ELISA Kit for Vinculin (VCL)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB839Mu	Multiplex Assay Kit for Vinculin (VCL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.