Packages (Simulation)

Polyclonal Antibody to Complement Factor I (CFI)

Image (I)

DAB staining on IHC-P; Sample: Mouse Colon Tissue Primary Ab: 10µg/ml Rabbit Anti-Mouse CFI Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse CFI Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Lung Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse CFI Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant CFI, Mouse.
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse CFI Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse CFI Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Small intestine lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse CFI Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected

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Instruction Manual

Polyclonal-Antibody-to-Complement-Factor-I-(CFI)-PAB978Mu01.pdf

Certificate

Polyclonal Antibody to Complement Factor I (CFI)

CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator

Product No.PAB978Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB978Mu01-Recombinant Complement Factor I (CFI)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 103 239 US$ 239 US$ 342 US$ 855 US$ 3420
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CFI. It has been selected for its ability to recognize CFI in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
J Thromb Haemost	A case report of thrombotic microangiopathy without renal involvement carrying two novel mutations in complement-regulator genes PubMed: 26613809
Pediatr Int	Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527
United States Patent Application	NOVEL ASSAY y2016:139118
Researchgate	Compliment factor i bioassay US20170285024
PLoS One	A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and … Pubmed:29782502
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE	The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H 33788378

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPB978Mu01	Recombinant Complement Factor I (CFI)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB978Mu01	Polyclonal Antibody to Complement Factor I (CFI)	WB; IHC; ICC; IP.