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Polyclonal Antibody to Cytochrome P450 17A1 (CYP17A1)

Image (I)

DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat S17aH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Adrenal gland Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat S17aH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat CYP17A1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Adrenal gland Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat CYP17A1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant S17aH, Rat.
Western Blot; Samples: Lane1: Rat Testis lysate; Lane2: A549 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat CYP17A1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cytochrome-P450-17A1-(CYP17A1)-PAC016Ra01.pdf

Certificate

Polyclonal Antibody to Cytochrome P450 17A1 (CYP17A1)

S17aH; CPT7; P450C17; 17-Alpha-Hydroxylase; 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450,Family 17,Subfamily A,Polypeptide 1

Product No.PAC016Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC016Ra01-Recombinant Cytochrome P450 17A1 (CYP17A1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreHomo sapiens (Human)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 114 266 US$ 266 US$ 380 US$ 950 US$ 3800
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CYP17A1. It has been selected for its ability to recognize CYP17A1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPC016Ra01	Recombinant Cytochrome P450 17A1 (CYP17A1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC016Ra01	Polyclonal Antibody to Cytochrome P450 17A1 (CYP17A1)	WB; IHC; ICC; IP.