Packages (Simulation)

Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDPK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human PDPK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human PDPK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant PDPK1, Human.
Western Blot; Sample: MCF7 cell lysate
Primary Ab: 0.5µg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Phosphoinositide-Dependent-Protein-Kinase-1-(PDPK1)-PAC246Hu01.pdf

Certificate

Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)

PDK1; PkB-Like 1

Product No.PAC246Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC246Hu01-Recombinant Phosphoinositide Dependent Protein Kinase 1 (PDPK1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.35mg/ml
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PDPK1. It has been selected for its ability to recognize PDPK1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Oncotarget	Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy Pubmed:29423092

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC246Hu01	Recombinant Phosphoinositide Dependent Protein Kinase 1 (PDPK1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC246Hu01	Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)	WB; IHC; ICC/IF