Packages (Simulation)
Image (I)
-
DAB staining on IHC-P; Samples: Mouse Adipose tissue Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
DAB staining on IHC-P;
Samples: Mouse Stomach Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Pancreas Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Uterus Tissue
Primary Ab: 30µg/ml Rabbit Anti-Mouse NPM1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 Cells;
Primary Ab: 10µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 7µg/ml Rabbit Anti-Mouse NPM1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Image (II)
-
Figure. Western Blot; Sample: Recombinant NPM, Mouse.
-
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Jurkat cell lysate; Lane3: K562 cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: NPM knockout Jurkat cell lysate;
Predicted MW: 33kd
Observed MW: 38kd
Primary Ab: 1µg/ml Rabbit Anti-Mouse NPM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
- KO-Validated
Polyclonal Antibody to Nucleophosmin 1 (NPM1)
B23; NPM; Nucleolar Phosphoprotein B23; Numatrin; Nucleophosmin/Nucleoplasmin Family,Member 1; Nucleolar protein NO38
- Product No.PAC664Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC664Mu02-Recombinant Nucleophosmin 1 (NPM1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 108
US$ 252
US$ 360
US$ 900
US$ 3600
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NPM1. It has been selected for its ability to recognize NPM1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
-
Antibody Labeling Customized Service
-
Protein A/G Purification Column
-
Staining Solution for Cells and Tissue
-
Positive Control for Antibody
-
Tissue/Sections Customized Service
-
Phosphorylated Antibody Customized Service
-
Western Blot (WB) Experiment Service
-
Immunohistochemistry (IHC) Experiment Service
-
Immunocytochemistry (ICC) Experiment Service
-
Flow Cytometry (FCM) Experiment Service
-
Immunoprecipitation (IP) Experiment Service
-
Immunofluorescence (IF) Experiment Service
-
Buffer
-
DAB Chromogen Kit
-
SABC Kit
-
Long-arm Biotin Labeling Kit
-
Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC664Mu01 | Recombinant Nucleophosmin 1 (NPM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC664Mu02 | Recombinant Nucleophosmin 1 (NPM1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC664Mu02 | Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB,IHC,ICC/IF |
| PAC664Mu01 | Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC; IP. |
| LAC664Mu71 | Biotin-Linked Polyclonal Antibody to Nucleophosmin 1 (NPM1) | WB; IHC; ICC. |
| SEC664Mu | ELISA Kit for Nucleophosmin 1 (NPM1) | Enzyme-linked immunosorbent assay for Antigen Detection. |