Packages (Simulation)

Polyclonal Antibody to Glycyl tRNA Synthetase (GARS)

Image (I)

DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human GARS Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant GARS, Human.
Western Blot; Sample: Porcine Stomach lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human GARS Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human GARS Polyclonal Antibody (Catalog PAC996Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Glycyl-tRNA-Synthetase-(GARS)-PAC996Hu01.pdf

Certificate

Polyclonal Antibody to Glycyl tRNA Synthetase (GARS)

CMT2D; CMT2-D; DSMAV; GlyRS; SMAD1; EJ; Charcot-Marie-Tooth Neuropathy 2D; Glycine tRNA Ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase

Product No.PAC996Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC996Hu01-Recombinant Glycyl tRNA Synthetase (GARS)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GARS. It has been selected for its ability to recognize GARS in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Oncotarget	Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme 33473259

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC996Hu01	Recombinant Glycyl tRNA Synthetase (GARS)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC996Hu01	Polyclonal Antibody to Glycyl tRNA Synthetase (GARS)	WB; IHC; ICC/IF; FCM
MAC996Hu22	Monoclonal Antibody to Glycyl tRNA Synthetase (GARS)	WB; IHC; ICC; IP.
SEC996Hu	ELISA Kit for Glycyl tRNA Synthetase (GARS)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEC996Hu	ELISA Kit for Anti-Glycyl tRNA Synthetase Antibody (Anti-GARS)	Enzyme-linked immunosorbent assay for Antibody Detection.
LMC996Hu	Multiplex Assay Kit for Glycyl tRNA Synthetase (GARS) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.