Packages (Simulation)

Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5)

Image (I)

DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 Cells;
Primary Ab: 20µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

WB Image
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human XRCC5 Polyclonal Antibody (Catalog PAD013Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-X-Ray-Repair-Cross-Complementing-5-(XRCC5)-PAD013Hu01.pdf

Certificate

Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5)

KARP1; KU80; KUB2; Ku86; NFIV; TLAA; Lupus Ku Autoantigen Protein P80; Nuclear factor IV; 86 kDa of Ku antigen; CTC box-binding factor 85 kDa; Thyroid-lupus autoantigen

Product No.PAD013Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD013Hu01-Recombinant X-Ray Repair Cross Complementing 5 (XRCC5)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against XRCC5. It has been selected for its ability to recognize XRCC5 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Am J Physiol Lung Cell Mol Physiol	Non-canonical role for Ku70/80 in the prevention of allergic airway inflammation via maintenance of airway epithelial cell organelle homeostasis Pubmed: 32877223

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPD013Hu01	Recombinant X-Ray Repair Cross Complementing 5 (XRCC5)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD013Hu01	Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5)	WB; IHC; ICC; IP; FCM
MAD013Hu21	Monoclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5)	WB; IHC; ICC; IP.
SED013Hu	ELISA Kit for X-Ray Repair Cross Complementing 5 (XRCC5)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD013Hu	Multiplex Assay Kit for X-Ray Repair Cross Complementing 5 (XRCC5) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSD013Hu01	ELISA Kit DIY Materials for X-Ray Repair Cross Complementing 5 (XRCC5)	Main materials for "Do It(ELISA Kit) Yourself"