Packages (Simulation)

Image (I)

IHC Images

Image (II)

Figure. Western Blot; Sample: Recombinant CCNB, Human.
Western Blot; Sample: Jurkat cell lysate
Primary Ab: 0.3µg/ml Rabbit Anti-Human CCNB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: CCNB knockout Jurkat cell lysate;
Predicted MW: 48,44kd
Observed MW: 55kd
Primary Ab: 1µg/ml Rabbit Anti-Human CCNB Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: K562 cell lysate; Lane2: 293T cell lysate;
Primary Ab: 0.4µg/ml Rabbit Anti-Human CCNB Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cyclin-B-(CCNB)-PAD264Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Cyclin B (CCNB)

CCNB1; CCN-B; G2/Mitotic-Specific Cyclin B1

Product No.PAD264Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD264Hu01-Recombinant Cyclin B (CCNB)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species Moren/a
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CCNB. It has been selected for its ability to recognize CCNB in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPD264Hu01	Recombinant Cyclin B (CCNB)	Positive Control; Immunogen; SDS-PAGE; WB.
PAD264Hu01	Polyclonal Antibody to Cyclin B (CCNB)	WB
MAD264Hu21	Monoclonal Antibody to Cyclin B (CCNB)	WB; IHC; ICC; IP.
SED264Hu	ELISA Kit for Cyclin B (CCNB)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD264Hu	Multiplex Assay Kit for Cyclin B (CCNB) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.