Packages (Simulation)

Polyclonal Antibody to Lactate Dehydrogenase C (LDHC)

Image (I)

DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat LDHC Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Testis Tissue
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat LDHC Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant LDHC, Rat.
Western Blot; Samples: Lane1: Rat Serum; Lane2: Rat Cerebrum lysate; Lane3: Rat Liver lysate; Lane4: Rat Testis lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat LDHC Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Lactate-Dehydrogenase-C-(LDHC)-PAE131Ra01.pdf

Certificate

Polyclonal Antibody to Lactate Dehydrogenase C (LDHC)

LDH-C; CT32; LDHX; LDH3; Cancer/Testis Antigen 32; LDH testis subunit

Product No.PAE131Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE131Ra01-Recombinant Lactate Dehydrogenase C (LDHC)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 114 266 US$ 266 US$ 380 US$ 950 US$ 3800
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LDHC. It has been selected for its ability to recognize LDHC in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Eur J Cancer.	High mobility group box 1 released from necrotic cells enhances regrowth and metastasis of cancer cells that have survived chemotherapy. Pubmed: 23040637
American Journal of Translational Research	Clusterin signals via ApoER2/VLDLR and induces meiosis of male germ cells. pubmed:28386352

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPE131Ra01	Recombinant Lactate Dehydrogenase C (LDHC)	Positive Control; Immunogen; SDS-PAGE; WB.
APE131Ra01	Active Lactate Dehydrogenase C (LDHC)	Cell culture; Activity Assays.
PAE131Ra01	Polyclonal Antibody to Lactate Dehydrogenase C (LDHC)	WB; IHC; ICC; IP.
MAE131Ra21	Monoclonal Antibody to Lactate Dehydrogenase C (LDHC)	WB; IHC; ICC; IP.
SEE131Ra	ELISA Kit for Lactate Dehydrogenase C (LDHC)	Enzyme-linked immunosorbent assay for Antigen Detection.
LME131Ra	Multiplex Assay Kit for Lactate Dehydrogenase C (LDHC) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.