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Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)

Image (I)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human SPHK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human SPHK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant SPHK1, Human.
Figure. Western Blot; Lane1: Human Raji Tissue; Lane2: Mouse Lung Tissue.
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human SPHK1 Polyclonal Antibody (Catalog # PAE698Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

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Instruction Manual

Polyclonal-Antibody-to-Sphingosine-Kinase-1-(SPHK1)-PAE698Hu01.pdf

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Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)

Product No.PAE698Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE698Hu01-Recombinant Sphingosine Kinase 1 (SPHK1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SPHK1. It has been selected for its ability to recognize SPHK1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPE698Hu01	Recombinant Sphingosine Kinase 1 (SPHK1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAE698Hu01	Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)	WB; IHC
FAE698Hu01	Anti-Sphingosine Kinase 1 (SPHK1) Polyclonal Antibody	Flow cytometry.
MAE698Hu21	Monoclonal Antibody to Sphingosine Kinase 1 (SPHK1)	WB; IHC; ICC; IP.
SEE698Hu	ELISA Kit for Sphingosine Kinase 1 (SPHK1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LME698Hu	Multiplex Assay Kit for Sphingosine Kinase 1 (SPHK1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.