Packages (Simulation)

Polyclonal Antibody to Betaine Homocysteine Methyltransferase (BHMT)

Image (I)

DAB staining on IHC-P; Samples: Porcine Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human BHMT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Porcine Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

WB Image
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human BHMT Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human BHMT Polyclonal Antibody (Catalog PAG464Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Betaine-Homocysteine-Methyltransferase-(BHMT)-PAG464Hu01.pdf

Certificate

Polyclonal Antibody to Betaine Homocysteine Methyltransferase (BHMT)

Betaine—homocysteine S-methyltransferase

Product No.PAG464Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPG464Hu01-Recombinant Betaine Homocysteine Methyltransferase (BHMT)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against BHMT. It has been selected for its ability to recognize BHMT in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPG464Hu01	Recombinant Betaine Homocysteine Methyltransferase (BHMT)	Positive Control; Immunogen; SDS-PAGE; WB.
PAG464Hu01	Polyclonal Antibody to Betaine Homocysteine Methyltransferase (BHMT)	WB; IHC; ICC; IP; FCM
MAG464Hu21	Monoclonal Antibody to Betaine Homocysteine Methyltransferase (BHMT)	WB; IHC; ICC; IP.
SEG464Hu	ELISA Kit for Betaine Homocysteine Methyltransferase (BHMT)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMG464Hu	Multiplex Assay Kit for Betaine Homocysteine Methyltransferase (BHMT) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.