Packages (Simulation)
Image (I)
-
DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Brain Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Figure: FITC staining on IHC-P;
Sample: A549 cells.
Image (II)
-
Figure. Western Blot; Sample: Recombinant UCHL1, Human.
-
Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Porcine Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human A549 Cells.
-
Human SH-SY5Y cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human UCHL1 Polyclonal Antibody (Catalog PAC030Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- Product No.PAG945Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPG945Hu01-Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against UCHL1. It has been selected for its ability to recognize UCHL1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
-
Antibody Labeling Customized Service
-
Protein A/G Purification Column
-
Staining Solution for Cells and Tissue
-
Positive Control for Antibody
-
Tissue/Sections Customized Service
-
Phosphorylated Antibody Customized Service
-
Western Blot (WB) Experiment Service
-
Immunohistochemistry (IHC) Experiment Service
-
Immunocytochemistry (ICC) Experiment Service
-
Flow Cytometry (FCM) Experiment Service
-
Immunoprecipitation (IP) Experiment Service
-
Immunofluorescence (IF) Experiment Service
-
Buffer
-
DAB Chromogen Kit
-
SABC Kit
-
Long-arm Biotin Labeling Kit
-
Real Time PCR Experimental Service
| Magazine | Citations |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions PubMed: 26232084 |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome Pubmed:26810533 |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135 |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes pubmed:28667290 |
| Intensive Care Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability Pubmed:29523900 |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury article:10.1088 |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage ISBN:978-952-03-0750-9 | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness Pubmed: 31896448 |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma Pubmed: 32987792 |