Packages (Simulation)

Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)

Image (I)

IHC Images

Image (II)

Figure. Western Blot; Sample: Recombinant PRAME, Human.
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human PRAME Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human PRAME Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human PRAME Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Testis lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human PRAME Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Preferentially-Expressed-Antigen-In-Melanoma-(PRAME)-PAH323Hu02.pdf

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Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)

MAPE; OIP4; CT130; Cancer/Testis Antigen 130; Opa-interacting protein 4; Melanoma antigen preferentially expressed in tumors

Product No.PAH323Hu02
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPH323Hu02-Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PRAME. It has been selected for its ability to recognize PRAME in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPH323Hu01	Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)	Positive Control; Immunogen; SDS-PAGE; WB.
RPH323Hu02	Recombinant Preferentially Expressed Antigen In Melanoma (PRAME)	Positive Control; Immunogen; SDS-PAGE; WB.
PAH323Hu01	Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)	WB; IHC
PAH323Hu02	Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)	WB; IHC; ICC; IP.
SEH323Hu	ELISA Kit for Preferentially Expressed Antigen In Melanoma (PRAME)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMH323Hu	Multiplex Assay Kit for Preferentially Expressed Antigen In Melanoma (PRAME) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSH323Hu01	ELISA Kit DIY Materials for Preferentially Expressed Antigen In Melanoma (PRAME)	Main materials for "Do It (ELISA Kit) Yourself".