Packages (Simulation)

Image (I)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human PPL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PPL Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

WB Image
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human PPL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Skin lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human PPL Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Periplakin--PPL--PAH334Hu01.pdf

Certificate

Polyclonal Antibody to Periplakin (PPL)

190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein

Product No.PAH334Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPH334Hu01-Recombinant Periplakin (PPL)
Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PPL. It has been selected for its ability to recognize PPL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPH334Hu02	Recombinant Periplakin (PPL)	Positive Control; Immunogen; SDS-PAGE; WB.
RPH334Hu01	Recombinant Periplakin (PPL)	Positive Control; Immunogen; SDS-PAGE; WB.
PAH334Hu01	Polyclonal Antibody to Periplakin (PPL)	WB; IHC; ICC; IP.
PAH334Hu02	Polyclonal Antibody to Periplakin (PPL)	WB; IHC; ICC; IP.
SEH334Hu	ELISA Kit for Periplakin (PPL)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMH334Hu	Multiplex Assay Kit for Periplakin (PPL) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSH334Hu01	ELISA Kit DIY Materials for Periplakin (PPL)	Main materials for "Do It (ELISA Kit) Yourself".