Packages (Simulation)

Polyclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2)

Image (I)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human IDH2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant IDH2, Human.
Western Blot; Samples: Lane1: Porcine Heart lysate; Lane2: Jurkat cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: IDH2 knockout Jurkat cell lysate;
Predicted MW: 51,45kDa
Observed MW: 45kDa
Primary Ab: 1µg/ml Rabbit Anti-Human IDH2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Isocitrate-Dehydrogenase-2--mitochondrial-(IDH2)-PAH838Hu01.pdf

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KO-Validated

Polyclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2)

IDH; IDP; ICD-M; IDHM; mNADP-IDH; Isocitrate dehydrogenase [NADP], mitochondrial; Oxalosuccinate decarboxylase

Product No.PAH838Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPH838Hu01-Recombinant Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IDH2. It has been selected for its ability to recognize IDH2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Molecular Oncology	IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer Pubmed:29465809

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
APH838Hu01	Active Isocitrate Dehydrogenase 2, mitochondrial (IDH2)	Cell culture; Activity Assays.
RPH838Hu01	Recombinant Isocitrate Dehydrogenase 2, mitochondrial (IDH2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAH838Hu01	Polyclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2)	WB,IHC
MAH838Hu21	Monoclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2)	WB; IHC; ICC; IP.
SEH838Hu	ELISA Kit for Isocitrate Dehydrogenase 2, mitochondrial (IDH2)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMH838Hu	Multiplex Assay Kit for Isocitrate Dehydrogenase 2, mitochondrial (IDH2) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.