Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human ACO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Figure. Western Blot; Sample: Recombinant ACO1, Human.
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Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human SiHa cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ACO1 Polyclonal Antibody (Catalog PAJ683Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Instruction Manual
Certificate
Polyclonal Antibody to Aconitase 1 (ACO1)
IREB1; IRP1; IREBP; Iron regulatory protein 1; Iron-responsive element-binding protein 1; Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein
- Product No.PAJ683Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPJ683Hu02-Recombinant Aconitase 1 (ACO1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
- ApplicationsWB; ICC; IHC-P; IHC-F; ELISA; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ACO1. It has been selected for its ability to recognize ACO1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 1-5μg/mL
Immunocytochemistry in formalin fixed cells: 5-20μg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20μg/mL
Immunohistochemistry in paraffin section: 5-20μg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPJ683Hu02 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu03 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu04 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu01 | Recombinant Aconitase 1 (ACO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ683Hu04 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; ICC; IHC-P; IHC-F; ELISA. |
| PAJ683Hu02 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; ICC; IHC-P; IHC-F; ELISA; FCM |
| PAJ683Hu01 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; ICC; IHC-P; IHC-F; ELISA; FCM |
| PAJ683Hu03 | Polyclonal Antibody to Aconitase 1 (ACO1) | WB; IHC; ICC; IP; FCM |
| LAJ683Hu71 | Biotin-Linked Polyclonal Antibody to Aconitase 1 (ACO1) | WB; IHC; ICC. |
| SEJ683Hu | ELISA Kit for Aconitase 1 (ACO1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ683Hu | Multiplex Assay Kit for Aconitase 1 (ACO1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |