Packages (Simulation)

Polyclonal Antibody to Programmed Cell Death Protein 6 (PDCD6)

Image (I)

FITC staining on IF; Samples: Human Hela cell; Primary Ab: 20µg/ml Rabbit Anti-Human PDCD6 Antibody Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PDCD6 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant PDCD6, Human.
Western Blot; Sample: Hela cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Human PDCD6 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: PDCD6 knockout Hela cell lysate;
Predicted MW: 22,7kd
Observed MW: 23kd
Primary Ab: 1µg/ml Rabbit Anti-Human PDCD6 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PDCD6 Polyclonal Antibody (Catalog PAL096Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Programmed-Cell-Death-Protein-6-(PDCD6)-PAL096Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Programmed Cell Death Protein 6 (PDCD6)

ALG-2; PEF1B; Apoptosis-Linked Gene-2; Probable calcium-binding protein ALG-2

Product No.PAL096Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPL096Hu01-Recombinant Programmed Cell Death Protein 6 (PDCD6)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116 270 US$ 270 US$ 386 US$ 965 US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PDCD6. It has been selected for its ability to recognize PDCD6 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPL096Hu01	Recombinant Programmed Cell Death Protein 6 (PDCD6)	Positive Control; Immunogen; SDS-PAGE; WB.
PAL096Hu01	Polyclonal Antibody to Programmed Cell Death Protein 6 (PDCD6)	WB; IHC; ICC/IF; FCM
MAL096Hu21	Monoclonal Antibody to Programmed Cell Death Protein 6 (PDCD6)	WB; IHC; ICC; IP.
SEL096Hu	ELISA Kit for Programmed Cell Death Protein 6 (PDCD6)	Enzyme-linked immunosorbent assay for Antigen Detection.
LML096Hu	Multiplex Assay Kit for Programmed Cell Death Protein 6 (PDCD6) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.