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Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 1 (WNT1)

INT1; Proto-oncogene Int-1 homolog

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against WNT1. It has been selected for its ability to recognize WNT1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Oncotarget Docosahexaenoic acid inhibits 12-O-tetradecanoylphorbol-13- acetate-induced fascin-1-dependent breast cancer cell migration by suppressing the PKCδ- and Wnt-1/β-catenin-mediated pathways Pubmed:27036017
Journal of Molecular and Cellular Cardiology Wnt1 inhibits vascular smooth muscle cell calcification by promoting ANKH expression Pubmed: 31356809
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