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Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)

Image (I)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NQO1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure: FITC staining on IHC-P;
Sample: A549 cells.

Image (II)

Figure. Western Blot; Sample: Recombinant NQO1, Human.
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human NQO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human NQO1 Polyclonal Antibody (Catalog PAL969Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-NADH-Dehydrogenase--Quinone-1-(NQO1)-PAL969Hu01.pdf

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Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)

DHQU; DIA4; DTD; NMOR1; NMORI; QR1; Azoreductase; DT-diaphorase; Menadione reductase; Phylloquinone reductase; Quinone reductase 1; NAD(P)H:quinone oxidoreductase 1

Product No.PAL969Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPL969Hu01-Recombinant NADH Dehydrogenase, Quinone 1 (NQO1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 116 270 US$ 270 US$ 386 US$ 965 US$ 3860
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NQO1. It has been selected for its ability to recognize NQO1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Hypertension	Transcription factor Nrf2 protects renal dopamine D1 receptor function during oxidative stress. Pubmed: 23876469
Free Radical Research	Notoginsenoside R1-mediated neuroprotection involves estrogen receptor-dependent crosstalk between Akt and ERK1/2 pathways: A novel mechanism of Nrf2/ARE signaling activation Informahealthcare: Source
physiological reports	Antioxidant resveratrol restores renal sodium transport regulation in SHR PubMed: 26603454
Biol Chem	Genetic association of NAD (P) H Quinone Oxidoreductase (NQO1* 2) polymorphism with NQO1 levels and risk of diabetic nephropathy Pubmed:27078674
Experimental Parasitology	Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazole Pubmed: 30790573
International Journal of Nanomedicine	Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions Pubmed: 32764932
Exp Parasitol	In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism Pubmed:35594934

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPL969Hu01	Recombinant NADH Dehydrogenase, Quinone 1 (NQO1)	Positive Control; Immunogen; SDS-PAGE; WB.
APL969Hu01	Active NADH Dehydrogenase, Quinone 1 (NQO1)	Cell culture; Activity Assays.
PAL969Hu01	Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)	WB; IHC; ICC; IP; FCM
MAL969Hu21	Monoclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)	WB; IHC; ICC; IP.
SEL969Hu	ELISA Kit for NADH Dehydrogenase, Quinone 1 (NQO1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LML969Hu	Multiplex Assay Kit for NADH Dehydrogenase, Quinone 1 (NQO1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.