Packages (Simulation)
Image (I)
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Briefly, recombinant TFPI antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to recombinant human TFPI-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with HRP labelled secondary antibody wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of rh TFPI and recombinant TFPI antibody was shown in Figure 1, and this effect was in a dose dependent manner.
Quality Guarantee
Certificate
Recombinant Antibody to Tissue Factor Pathway Inhibitor (TFPI)
TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor
- Product No.RAA394Hu64
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceRecombinant monoclonal antibody preparation
- HostE.coli
- Potency (Clone Number)n/a
- Ig Isotype IgG
- PurificationProtein A + Protein G affinity chromatography
- LabelCy3
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IF; ICC; IHC; IP; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 228
US$ 532
US$ 760
US$ 1900
US$ 7600
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against TFPI. It has been selected for its ability to recognize TFPI in immunohistochemical staining.
USAGE
Immunocytochemistry: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Mouse Antibody Variable Region Gene Amplification Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| South African Journal of Child Health | Tissue factor pathway inhibitor in paediatric patients with nephrotic syndrome Sajch: 383 |
| the journal of immunology | Cathepsin G in Experimental Tuberculosis: Relevance for Antibacterial Protection and Potential for Immunotherapy PubMed: 26320257 |
| Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502 |
| Molecular Carcinogenesis | Endothelial cell‐anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice PubMed: 25945811 |
| Cell Biology International | Extracellular vesicles from malignant effusions induce tumor cell migration: inhibitory effect ofLMWH tinzaparin. pubmed:27435911 |
| International Journal of Infectious Diseases | Kunitz-type protease inhibitor as a vaccine candidate against schistosomiasis mansoni pubmed:29128645 |