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Recombinant Caspase 14 (CASP14)

Caspase 14 Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 14

  • Product No.RPA457Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Cytoplasm
  • Predicted Molecular Mass31.4kDa
  • Accurate Molecular Mass31kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Gln242 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.1
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 178  US$ 446  US$ 892  US$ 2676  US$ 6690 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Dermatological Science Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis Pubmed:25315296
In Vitro Cell Dev Biol Anim Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes PubMed: 26427706
Applied Biochemistry and Biotechnology Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14 Pubmed: 30390204
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