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Recombinant Endothelin Converting Enzyme 1 (ECE1)

  • Product No.RPA483Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass32.7kDa
  • Accurate Molecular Mass30kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu469~Glu721 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point6.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 168  US$ 420  US$ 840  US$ 2520  US$ 6300 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Placenta Carbon monoxide attenuates bacteria-induced Endothelin-1 expression in second trimester placental explants Pubmed: 24731730
The Journal of Heart and Lung Transplantation The Role of the Endothelin-1 Pathway as a Biomarker for Donor Lung Assessment in Clinical Ex Vivo Lung Perfusion Sciencedirect:S1053249815000054
J Neurochem. Endothelin-converting enzymes degrade α-synuclein and are reduced in dementia with Lewy bodies. pubmed:28171705
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA483Hu01 Recombinant Endothelin Converting Enzyme 1 (ECE1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA483Hu01 Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) WB; IHC; ICC; IP.
MAA483Hu22 Monoclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) WB; IHC; ICC; IP.
SEA483Hu ELISA Kit for Endothelin Converting Enzyme 1 (ECE1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA483Hu Multiplex Assay Kit for Endothelin Converting Enzyme 1 (ECE1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.