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Recombinant Cytochrome P450 27B1 (CYP27B1)

VDD1, PDDR; CYP1, P450c1; 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase; Cytochrome P450 Family 27 Subfamily B Polypeptide 1; Calcidiol 1-monooxygenase; VD3 1A hydroxylase

  • Product No.RPD539Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass31.8kDa
  • Accurate Molecular Mass32kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp256~Arg508 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point6.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 182  US$ 456  US$ 912  US$ 2736  US$ 6840 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Bone and Mineral Research Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1α‐Hydroxylase Expression in Vascular Smooth Muscle Cells Pubmed:27074284
Nutrients Lead Affects Vitamin D Metabolism in Rats Pubmed:29495376
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD539Hu01 Recombinant Cytochrome P450 27B1 (CYP27B1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD539Hu01 Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1) WB; IHC; ICC; IP.
SED539Hu ELISA Kit for Cytochrome P450 27B1 (CYP27B1) Enzyme-linked immunosorbent assay for Antigen Detection.