It is well known that after knowing the gene sequence of the protein, the gene can be cloned into the plasmid. The plasmid can be transfected into E. coli, then the E.coli is the factory for protein expression. After separation and purification, the recombinant protein can be gotten. E. coli expression system is on of the expressions for protein expression. Eukaryotic expressin systems also can be used in protein expression. Figure 1 showed the expression of recombinant protein.Cloud-Clone Crop. provides one-stop services from genes to proteins. Customers also can choose one or two kinds of services according to their specific requirements. There are four protein expressions platform i......2017-05-19

Hyaluronic acid (HA) is one of the main components of the extracellular matrix, it is an anionic small molecule. HA is hydrolyzed by HAase in vivo, liver is the site of metabolism. Molecular formula of HA is (C14H21NO11)n, the molecular weight of HA ranges from 5kDa to 20,000kDa, the structural formula of HA as shown in figure 1.The proliferation and differentiation of epidermal cells can be promoted by HA, besides, HA has the oxygen free-radical scavenging property, these contribute to the restoration of skin damage. Moreover, HA is known as the ideal natural moisturizing factor because of its water-retaining property. You must be familiar with the name of hyaluronic acid, especially ......2017-05-19

We have introduced cell autophagy marker LC3 before, and now let’s talk about the new marker VCP, which mediates cell endocytosis and autophagy.Valosin Containing Protein (VCP) is a membrane-associated glycoprotein associated with multicellular activity, and its two tandem ATPase domains (named D1 and D2) are shown in Fig 1. As a class of like-chaperones, VCP can play an important role in the endoplasmic reticulum-associated protein degradation and cell cycle regulation, mediating intracellular transport and autophagy. After combination of VCP and its cofactor UFD1-NPL4, ubiquitination-folding proteins could happen degradation.Fig1. Schematic diagram of VCP domain structure (from wik......2017-04-14

Antibody preparation technologies have gone three stages of development. The first stage: antiserum preparation or polyclonal antibody preparation by immunizing higher vertebrates. And the second stage: monoclonal antibody preparation based on cell fusion technologies and discovery of mouse myeloma; the third stage: recombinant antibody preparation by gene sequencing and gene library screening technologies. The polyclonal preparation technology can’t be suitable for large-scale production of antibody as to the complex ingredient and poor application of polyclonal Ab. The monoclonal antibody preparation technology is a combination of hybridoma technology and fermentation engineering t......2017-04-14

Microcarrier Cell Culture Technology allows a high yield culture of anchorage-dependent cells. The microcarriers can be maintained in suspension with gently stirring. And cells grow as monolayers on the surface of microcarries. Microcrriers are typically 60-250 micrometre spheres and characterized by non-toxicity and uniform density. The microcarriers are commonly used in the large-scale cell culture for high-yield commercial production of vaccine, genetic engineering drugs and so on, since cells grow fig.1. Application of microcarrier cellas monolayers on the surface of microcarrier culture technology in Ab production.to enlarge the attachment surface of cell growth.It is impossible......2017-04-14

As we all know, internal control proteins are expressed by house keeping genes. These proteins show relatively constant quantity in different tissues and cells. When we arranged an experiment to test the expression of target proteins, they could be considered as a reference in the experiment.The internal control proteins often are used in the Western Blot. Western Blot can not only be used to prove whether the samples contain the target protein, it also can be used for discriminating the difference of protein quantity among different samples and different conditions. So Western Blot is usually designed for protein expression level test. Internal control proteins as a reference, are use......2017-04-14