Rheumatoid arthritis is a chronic autoimmune disease that is associated with complex inflammatory mediators, which will cause joint injury,synovial inflammation, cartilage and bone damage.The typical characteristic of this chronic disease is the chronic joint injury, seriously affecting people's health and the development of animal husbandry. The incidence of RA is generally believed to be caused by multiple factors,which include genetic factors,bacteria and virus mediated infection factors,autoimmune tolerance damage, endocrine disorders and other factors of cold,damp, fatigue, trauma, smoking and mental stimulation.The pathological symptoms were mainly manifested in the aspects o......2016-06-12

Chemiluminescence Enzyme Immunoassay is a kind of detection method which combined magnetic separation technology, sensitive chemiluminescence analysis with specific antigen-antibody reaction. For sandwich chemiluminescence enzyme immunoassay, the complex of enzyme labeled antibody, target antigen in samples and magnetic particle antibody act as a “sandwich” structure. And then the complex are segregated from other materials and unbound enzyme conjugated antibody. After that, the luminescent substrate is added to generate glow light emission kinetics. The target antigen can be measured qualitatively as the luminous intensity is proportional to the target antigen level in the sample ......2016-06-12

We had a brife introduction about the cascade activation signal pathway of MAPK in a early topic, including extracellular signal-regulated protein kinase(ERK), c-Jun N-terminal kinase(JNK), P38MAPK and ERK5. Among them, the pathways of JNK, P38MAPK and ERK5 belong to stress activated MAPK pathways. The discovery of ERK5 pathway is relatively late, but its research is very active.ERK5 includes 816 amino acids, and its length is almost 2 times of other members of the MAPK family. N-terminal of ERK5 has a domain of kinase, and C-terminal has a unique COOH, which is obviously different from the other members of the MAPK family. It is a three-dimensional structure of ERK5 shown in Figure 1.......2016-06-12

With thedevelopment of science and technology, protein structure and metabolism studybegan rapid development, especially in recent years. The NMR (Nuclear Magnetic Resonance)technology has been widely used in the field of protein structure,which becamesimple and feasible to analyze the structure of the protein. The NMR technologyof the basic principle for analyzing thestructure of the protein is that . the nuclear mass spectrometer issued aseries of electromagnetic wave, stimulating H, N and C atoms, the H, N, C atomsfrom the ground state transition into unstable excited state;when theelectromagnetic wave stopped, excited states of atoms will automatically returnto the ground state, an......2016-05-12

GlucosidaseAlpha, Acid (GaA) is a kind of amylum hydrolase enzyme, which catalyzes thehydrolysis of polysaccharides to produce alpha D- glucose. In addition, it alsopossesses transglycosylation activity, which can transfer the alpha -1,4-glycosidic bond to alpha-1,6- glycosidic bond or other form linkages, to obtainnonfermented isomalto- oligosaccharide, glycolipids and glycopeptide etc.. The gene,which encode the GAA, is located at chromosome 17. Defects in this gene maylead to deficiency of GaA and glycogen accumulation in skeletal muscle, smoothmuscle & cardiac muscle tissue as the glycogen can’t be hydrolyzed by GaA. Then it may cause severeneuromuscular disease. This lysosomal s......2016-05-12

Complements are mainly distributed inbody fluids and cell surface of human or animal. After activation, they couldmediate immune and inflammatory responses, they are also known as thecomplement system.Complement system consists of nearly 40kinds of ingredients, most of them are glycoproteins.There are 13 inherentcomponents, over 10 regulatory proteins, and more than 10 kinds of complementreceptors.Complementcan’t be saved in hot temperature,when it reaches 56 degrees, most of the complementswill lose the activity after 30 minutes. Complement is difficult to preserve,in freeze and dry environment, they can bestored in relatively long time.ComplementComponent 3(C3) is a core component.......2016-05-12