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Western Blot; Sample: Hela cell lysate
Primary Ab: 0.2µg/ml Biotin-Linked Polyclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)
Second Ab: 0.2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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Biotin-Linked Polyclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)
CD156b; TACE; CSVP; Tumor Necrosis Factor,Alpha,Converting Enzyme; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease
- Product No.LAB555Hu71
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 86
US$ 202
US$ 288
US$ 720
US$ 2880
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ADAM17. It has been selected for its ability to recognize ADAM17 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748 |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748 |
| Biomedical Research | ADAM17 regulates TNF-α expression upon lipopolysaccharide stimulation in oral keratinocytes. pubmed:28637950 |
| Cytometry B Clin Cytom | L‐Selectin Expression Is Influenced by Phosphatase Activity in Chronic Lymphocytic Leukemia Pubmed: 30729673 |
| Disease Markers | Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100 |
| Chem Biol Interact | PEG-SOD attenuates the mitogenic ERK1/2 signaling cascade induced by cyclosporin A in the liver and kidney of albino mice Pubmed: 32866465 |
| life | High Levels of TNF-¦Á and TIM-3 as a Biomarker of Immune Reconstitution Inflammatory Syndrome in People with HIV Infection |