Packages (Simulation)

Monoclonal Antibody to Cytokeratin 4 (CK4)

Image (I)

DAB staining on IHC-P;
Sample: Porcine Uterus Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Lung Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue
Primary Ab: 30µg/ml Mouse Anti-Rat CK4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Image (II)

Figure.Western Blot;Sample: Recombinant KRT4, Rat.
Western Blot; Samples: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate;
Primary Ab: 0.2µg/ml Mouse Anti-Rat CK4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

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Instruction Manual

Monoclonal-Antibody-to-Keratin-4-(KRT4)-MAA489Ra21.pdf

Certificate

Monoclonal Antibody to Cytokeratin 4 (CK4)

CYK4; KRT4; K4; Keratin 4; Keratin, type II cytoskeletal 4; Type-II keratin Kb4

Product No.MAA489Ra21
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourceMonoclonal antibody preparation
HostMouse
Potency (Clone Number)1:270000 (1#)
Ig Isotype IgG1 Kappa
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPA489Ra01-Recombinant Cytokeratin 4 (CK4)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration1mg/mL
Organism Species MoreHomo sapiens (Human), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 109 253 US$ 253 US$ 362 US$ 905 US$ 3620
For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CK4. It has been selected for its ability to recognize CK4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPA489Ra01	Recombinant Cytokeratin 4 (CK4)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA489Ra01	Polyclonal Antibody to Cytokeratin 4 (CK4)	WB; IHC; ICC; IP.
LAA489Ra71	Biotin-Linked Polyclonal Antibody to Cytokeratin 4 (CK4)	WB; IHC; ICC.
MAA489Ra21	Monoclonal Antibody to Cytokeratin 4 (CK4)	WB; IHC; ICC; IP.