Packages (Simulation)
Image (I)
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IHC Images
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FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20µg/ml Mouse Anti-Human KPNa2 Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Mouse Anti-Human KPNa2 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
Image (II)
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Figure. Western Blot; Sample: Recombinant KPNa2, Human.
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Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2µg/ml Mouse Anti-Human KPNa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Raji cell lysate; Lane4: U2OS cell lysate
Primary Ab: 0.4µg/ml Mouse Anti-Human KPNa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human A549 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human KPNa2 Monoclonal Antibody (Catalog MAE748Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Instruction Manual
Certificate
Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2)
IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha
- Product No.MAE748Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C10
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPE748Hu01-Recombinant Karyopherin Alpha 2 (KPNa2)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/ml
- Organism Species Moren/a
- ApplicationsWB; ICC/IF;FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 108
US$ 252
US$ 360
US$ 900
US$ 3600
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against KPNa2. It has been selected for its ability to recognize KPNa2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunocytochemistry: 5-30µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Oncol Rep.? | KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferation Pubmed:25109899 |
| Tumor Biology | KPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosis pubmed:28651492 |
| Head & Neck | Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancer Pubmed:29542209 |
| International Journal of Cancer | Nuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers 34591985 |