Packages (Simulation)

Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)

Image (I)

DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse HSPA1A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebellum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Spinal cord Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: Mouse Kidney lysate; Lane3: Mouse Testis lysate Primary Ab: 0.1µg/ml Rabbit Anti-Mouse HSPA1A Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Samples: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Kidney lysate; Lane4: Mouse Testis lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Mouse HSPA1B Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
WB Image
Western Blot; Sample: A549 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Mouse HSPA1A Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected

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Instruction Manual

Polyclonal-Antibody-to-Heat-Shock-70kDa-Protein-1B-(HSPA1B)-PAA301Mu01.pdf

Certificate

Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)

HSP70-2; HSP-A1B; Heat Shock Protein 72

Product No.PAA301Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA301Mu01-Recombinant Heat Shock 70kDa Protein 1B (HSPA1B)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreHomo sapiens (Human)
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 97 227 US$ 227 US$ 324 US$ 810 US$ 3240
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HSPA1B. It has been selected for its ability to recognize HSPA1B in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPA301Mu01	Recombinant Heat Shock 70kDa Protein 1B (HSPA1B)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA301Mu01	Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)	WB; IHC; ICC/IF
LAA301Mu81	FITC-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)	WB; IHC; ICC; IF.
LAA301Mu71	Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)	WB; IHC; ICC.