Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Vector Red staining on IHC-P;
Samples: Human Placenta Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Prostate Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CD276 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Image (II)
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Figure. Western Blot; Sample: Recombinant CD276, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: PC3 cell lysate; Lane3: MCF7 cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: CD276 knockout 293T cell lysate;
Predicted MW: 57,53,33kd
Observed MW: 100kd
Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
ChoK1 transfected with human CD276 (Stable transfection) was stained with rabbit Anti-human CD276 Polyclonal Antibody (Catalog # PAA781Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Quality Guarantee
Instruction Manual
Certificate
- KO-Validated
Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule
- Product No.PAA781Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA781Hu01-Recombinant Cluster Of Differentiation 276 (CD276)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 95
US$ 221
US$ 316
US$ 790
US$ 3160
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CD276. It has been selected for its ability to recognize CD276 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
For flow cytometry: 0.5-2 µg per 10^6 cells in 100 µl volume;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA781Hu01 | Active Cluster Of Differentiation 276 (CD276) | Cell culture; Activity Assays. |
| RPA781Hu01 | Recombinant Cluster Of Differentiation 276 (CD276) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA781Hu01 | Polyclonal Antibody to Cluster Of Differentiation 276 (CD276) | WB; IHC; FCM |
| FAA781Hu01 | Anti-Cluster Of Differentiation 276 (CD276) Polyclonal Antibody | Flow cytometry. |
| MAA781Hu22 | Monoclonal Antibody to Cluster Of Differentiation 276 (CD276) | WB |
| MAA781Hu21 | Monoclonal Antibody to Cluster Of Differentiation 276 (CD276) | WB; IHC; ICC; IP. |
| SEA781Hu | ELISA Kit for Cluster Of Differentiation 276 (CD276) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA781Hu | Multiplex Assay Kit for Cluster Of Differentiation 276 (CD276) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |