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Human A431 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ITGb4 Polyclonal Antibody (Catalog PAB440Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Polyclonal Antibody to Integrin Beta 4 (ITGb4)
CD104; ITG-B4; GP150
- Product No.PAB440Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 101
US$ 236
US$ 337
US$ 843
US$ 3370
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ITGb4. It has been selected for its ability to recognize ITGb4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB440Hu01 | Recombinant Integrin Beta 4 (ITGb4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB440Hu02 | Polyclonal Antibody to Integrin Beta 4 (ITGb4) | WB; IHC; ICC; IP; FCM |
| PAB440Hu01 | Polyclonal Antibody to Integrin Beta 4 (ITGb4) | WB; IHC; ICC; IP. |
| MAB440Hu22 | Monoclonal Antibody to Integrin Beta 4 (ITGb4) | WB; IHC; ICC; IP. |
| SEB440Hu | ELISA Kit for Integrin Beta 4 (ITGb4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB440Hu | Multiplex Assay Kit for Integrin Beta 4 (ITGb4) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |