Packages (Simulation)

Polyclonal Antibody to Interferon Regulatory Factor 8 (IRF8)

Image (I)

Used in DAB staining on fromalin fixed paraffin- embedded Kidney tissue

Image (II)

Figure. Western Blot; Sample: Recombinant IRF8, Human.
Western Blot; Sample: Human Raji cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human IRF8 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human IRF8 Polyclonal Antibody (Catalog # PAB776Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

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Instruction Manual

Polyclonal-Antibody-to-Interferon-Regulatory-Factor-8-(IRF8)-PAB776Hu01.pdf

Certificate

Polyclonal Antibody to Interferon Regulatory Factor 8 (IRF8)

H-ICSBP; ICS-BP; ICSBP1; Interferon Consensus Sequence-Binding Protein 1

Product No.PAB776Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB776Hu01-Recombinant Interferon Regulatory Factor 8 (IRF8)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IRF8. It has been selected for its ability to recognize IRF8 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB776Hu01	Recombinant Interferon Regulatory Factor 8 (IRF8)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB776Hu01	Polyclonal Antibody to Interferon Regulatory Factor 8 (IRF8)	WB; IHC; ICC; IP.
FAB776Hu01	Anti-Interferon Regulatory Factor 8 (IRF8) Polyclonal Antibody	Flow cytometry.