Packages (Simulation)

Polyclonal Antibody to SMAD family member 5 (SMAD5)

Image (I)

DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human SMAD5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human SMAD5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant Smad5, Human.
Western Blot; Samples: Lane1: K562 cell lysate; Lane2: Hela cell lysate; Lane3: Jurkat cell lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human SMAD5 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-SMAD-family-member-5-(SMAD5)-PAC122Hu01.pdf

Certificate

Polyclonal Antibody to SMAD family member 5 (SMAD5)

MADH5; Dwfc; JV5-1; Mothers Against Decapentaplegic Homolog 5

Product No.PAC122Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC122Hu01-Recombinant SMAD family member 5 (SMAD5)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.38mg/mL
Organism Species MoreMus musculus (Mouse)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SMAD5. It has been selected for its ability to recognize SMAD5 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC122Hu01	Recombinant SMAD family member 5 (SMAD5)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC122Hu01	Polyclonal Antibody to SMAD family member 5 (SMAD5)	WB; IHC; ICC; IP.
SEC122Hu	ELISA Kit for SMAD family member 5 (SMAD5)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC122Hu	Multiplex Assay Kit for SMAD family member 5 (SMAD5) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.
KSC122Hu01	ELISA Kit DIY Materials for SMAD family member 5 (SMAD5)	Main materials for "Do It (ELISA Kit) Yourself".