Packages (Simulation)

Polyclonal Antibody to Exportin 1 (XPO1)

Image (I)

DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Uterus Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymph node Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Pancreas Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spinal cord Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant XPo1, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: K562 cell lysate; Lane3: Human Lung lysate; Lane4: Porcine Small intestine lysate
Primary Ab: 1µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: XPo1 knockout Hela cell lysate;
Predicted MW: 123kd
Observed MW: 110kd
Primary Ab: 1µg/ml Rabbit Anti-Human XPO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human XPo1 Polyclonal Antibody (Catalog PAC664Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Exportin-1-(XPO1)-PAC258Hu01.pdf

Certificate

KO-Validated

Polyclonal Antibody to Exportin 1 (XPO1)

Exp1; CRM1 Homolog,Yeast; Chromosome region maintenance 1 protein homolog

Product No.PAC258Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC258Hu01-Recombinant Exportin 1 (XPO1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against XPO1. It has been selected for its ability to recognize XPO1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Clin Cancer Res	Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Clin Cancer Res	Inactivation of the p53–KLF8–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402
Cellular Oncology	Mahendra Seervi, S. Sumi, Aneesh Chandrasekharan, Abhay K. Sharma & Doi: 10.1007/s13402-019-00451-1
Cellular Oncology	Molecular profiling of anastatic cancer cells: potential role of the nuclear export pathway Pubmed: 31147963
Journal of Cardiovascular Translational Research	XPO1 Gene Therapy Attenuates Cardiac Dysfunction in Rats with Chronic Induced Myocardial Infarction Pubmed: 31768947
Biol Chem	Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry 34472763

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC258Hu01	Recombinant Exportin 1 (XPO1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC258Hu01	Polyclonal Antibody to Exportin 1 (XPO1)	WB,IHC; FCM
FAC258Hu01	Anti-Exportin 1 (XPO1) Polyclonal Antibody	Flow cytometry.
FAC258Hu51	APC-Linked Anti-Exportin 1 (XPO1) Polyclonal Antibody	Flow cytometry.
MAC258Hu21	Monoclonal Antibody to Exportin 1 (XPO1)	WB; IHC; ICC; IP.
SEC258Hu	ELISA Kit for Exportin 1 (XPO1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC258Hu	Multiplex Assay Kit for Exportin 1 (XPO1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.