Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Sample: Porcine Colon Tissue Primary Ab: 10µg/ml Rabbit Anti-Human NUMA1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NUMA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NUMA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Pancreas Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human NUMA1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human NUMA1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Image (II)
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Figure. Western Blot; Sample: Recombinant NUMA1, Human.
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Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Human NUMA1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1)
NMP22; NUMA; Nuclear matrix protein-22; SP-H antigen
- Product No.PAC332Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPC332Hu01-Recombinant Nuclear Mitotic Apparatus Protein 1 (NUMA1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NUMA1. It has been selected for its ability to recognize NUMA1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Oncotarget | Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer inurine samples. pubmed:27203388 |
| Journal of Micromechanics and Microengineering | A simple fabricated microfluidic chip for urine sample-based bladder cancer detection |
| Journal of Electroanalytical Chemistry | A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample |
| Electrophoresis | Bladder cancer hunting: a microfluidic paper‐based analytical device Pubmed: 32530061 |