Packages (Simulation)

Polyclonal Antibody to Eukaryotic Translation Elongation Factor 2 (EEF2)

Image (I)

DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human EEF2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Uterine fibroids Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human EEF2 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

Figure. Western Blot; Sample: Recombinant EEF2, Human.
Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Human HepG2 Cells; Lane4: Human Raji Cells.
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human EEF2 Polyclonal Antibody (Catalog # PAC453Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

Quality Guarantee

Instruction Manual

Polyclonal-Antibody-to-Eukaryotic-Translation-Elongation-Factor-2-(EEF2)-PAC453Hu01.pdf

Certificate

Polyclonal Antibody to Eukaryotic Translation Elongation Factor 2 (EEF2)

EF2; Polypeptidyl-tRNA Translocase

Product No.PAC453Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC453Hu01-Recombinant Eukaryotic Translation Elongation Factor 2 (EEF2)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; IF;FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against EEF2. It has been selected for its ability to recognize EEF2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC453Hu01	Recombinant Eukaryotic Translation Elongation Factor 2 (EEF2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC453Hu01	Polyclonal Antibody to Eukaryotic Translation Elongation Factor 2 (EEF2)	WB; IHC; IF;FCM
FAC453Hu01	Anti-Eukaryotic Translation Elongation Factor 2 (EEF2) Polyclonal Antibody	Flow cytometry.