Packages (Simulation)

Polyclonal Antibody to Gastrokine 1 (GKN1)

Image (I)

DAB staining on IHC-P;
Sample: Porcine Stomach Tissue
Primary Ab: 1µg/ml Rabbit Anti-Porcine GKN1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant GKN1, Porcine.
Western Blot; Sample: Porcine Cerebrum lysate;
Primary Ab: 2µg/ml Rabbit Anti-Porcine GKN1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Porcine Stomach lysate; Lane2: Rat Stomach lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Porcine GKN1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Gastrokine-1--GKN1--pA92500Po01.pdf

Certificate

Polyclonal Antibody to Gastrokine 1 (GKN1)

CA11; AMP18; BRICD1; FOV; Foveolin; 18 kDa antrum mucosa protein

Product No.PAC500Po01
Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen n/a
Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreRattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 126 294 US$ 294 US$ 420 US$ 1050 US$ 4200
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GKN1. It has been selected for its ability to recognize GKN1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Jnournal of Cell Biochemistry	Gastrokine 1 regulates NF‐κB signaling pathway and cytokine expression in gastric cancers PubMed: 23444260
fedOA	Searching for informative biomarkers for Gastric Cancer id:eprint
Journal of Negative Results in BioMedicine	Gastrokine 1 mRNA in human sera is not informative biomarker for gastric cancer. pubmed:27452910

Catalog No.	Related products for research use of Sus scrofa; Porcine (Pig) Organism species	Applications (RESEARCH USE ONLY!)
RPC500Po01	Recombinant Gastrokine 1 (GKN1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC500Po01	Polyclonal Antibody to Gastrokine 1 (GKN1)	WB; IHC; ICC; IP.