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Polyclonal Antibody to Hemopoietic Cell Kinase (HCK)

Image (I)

DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human HCK Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lymph node Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human HCK Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant HCK, Human.
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human HCK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Placenta lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human HCK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Raji cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human HCK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human HCK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human HCK Polyclonal Antibody (Catalog # PAC522Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

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Instruction Manual

Polyclonal-Antibody-to-Hemopoietic-Cell-Kinase-(HCK)-PAC522Hu01.pdf

Certificate

Polyclonal Antibody to Hemopoietic Cell Kinase (HCK)

JTK9; Hematopoietic cell kinase; p59-HCK/p60-HCK

Product No.PAC522Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC522Hu01-Recombinant Hemopoietic Cell Kinase (HCK)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration1mg/ml
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HCK. It has been selected for its ability to recognize HCK in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPC522Hu01	Recombinant Hemopoietic Cell Kinase (HCK)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC522Hu01	Polyclonal Antibody to Hemopoietic Cell Kinase (HCK)	WB; IHC; ICC; IP.
FAC522Hu01	Anti-Hemopoietic Cell Kinase (HCK) Polyclonal Antibody	Flow cytometry.
SEC522Hu	ELISA Kit for Hemopoietic Cell Kinase (HCK)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC522Hu	Multiplex Assay Kit for Hemopoietic Cell Kinase (HCK) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.