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Polyclonal Antibody to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)

Image (I)

DAB staining on IHC-P; Samples: Mouse Intestine Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse MCL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Thyroid Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lymphoma Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human Hela cell;
Primary Ab: 40μg/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Image (II)

Figure. Western Blot; Sample: Recombinant MCL1, Mouse.
Western Blot; Sample: Mouse Spleen lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Small intestine lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-Mouse MCL1 Polyclonal Antibody (Catalog PAC615Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Induced-Myeloid-Leukemia-Cell-Differentiation-Protein-Mcl-1-(MCL1)-PAC615Mu01.pdf

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Polyclonal Antibody to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)

EAT; MCL1L; MCL1S; BCL2L3; TM; Myeloid Cell Leukemia Sequence 1, Bcl2 Related; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

Product No.PAC615Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC615Mu01-Recombinant Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreHomo sapiens (Human)
ApplicationsWB; IHC; ICC/IF;FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 108 252 US$ 252 US$ 360 US$ 900 US$ 3600
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MCL1. It has been selected for its ability to recognize MCL1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-40μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Cancers	Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia Pubmed: 31718075
ACTA NEUROPATHOLOGICA COMMUNICATIONS	Restoration of miR-193a expression is tumor-suppressive in MYC amplified Group 3 medulloblastoma Pubmed: 32410663
Cancers (Basel)	BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 33540760

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
RPC615Mu01	Recombinant Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)	Positive Control; Immunogen; SDS-PAGE; WB.
RPC615Mu02	Recombinant Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)	Positive Control; Immunogen; SDS-PAGE; WB.
EPC615Mu61	Eukaryotic Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAC615Mu01	Polyclonal Antibody to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)	WB; IHC; ICC/IF;FCM
PAC615Mu02	Polyclonal Antibody to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)	WB; IHC; ICC; IP.
SEC615Mu	ELISA Kit for Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMC615Mu	Multiplex Assay Kit for Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.