Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Sample: Human Placenta Tissue Primary Ab: 10µg/ml Rabbit Anti-Human EIF4A1 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Image (II)
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Western Blot; Sample: K562 cell lysate Primary Ab: 0.1µg/ml Rabbit Anti-Human EIF4A1 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Hela cell lysate; Lane3: MCF7 cell lysate; Lane4: NIH/3T3 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human EIF4A1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4A1 (EIF4A1)
DDX2A; EIF-4A; EIF4A; ATP-dependent RNA helicase eIF4A-1
- Product No.PAF009Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPF009Hu01-Recombinant Eukaryotic Translation Initiation Factor 4A1 (EIF4A1)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 105
US$ 245
US$ 350
US$ 875
US$ 3500
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against EIF4A1. It has been selected for its ability to recognize EIF4A1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;1:250-1000
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPF009Hu01 | Recombinant Eukaryotic Translation Initiation Factor 4A1 (EIF4A1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF009Hu01 | Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4A1 (EIF4A1) | WB; IHC; ICC; IP. |