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Recombinant Antibody to Protein Tyrosine Phosphatase Receptor Type C (CD45)

PTPRC; PTPR-C; LCA; B220; GP180; LY5; T200; Leukocyte common antigen

SPECIFITY

The antibody is a mouse monoclonal antibody raised against CD45. It has been selected for its ability to recognize CD45 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Flow cytometry: 0.2-2 µL/106 cells
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Cell Size-dependent segregation controls macrophage phagocytosis of antibody-opsonized targets Pubmed:29958103
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