≡

Recombinant Cytochrome P450 27B1 (CYP27B1)

VDD1, PDDR; CYP1, P450c1; 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase; Cytochrome P450 Family 27 Subfamily B Polypeptide 1; Calcidiol 1-monooxygenase; VD3 1A hydroxylase

  • Product No.RPD539Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass31.5kDa
  • Accurate Molecular Mass32kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp251~Asp500 with N-terminal His Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.7
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 194  US$ 486  US$ 972  US$ 2916  US$ 7290 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Bone and Mineral Research Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1α‐Hydroxylase Expression in Vascular Smooth Muscle Cells Pubmed:27074284
Nutrients Lead Affects Vitamin D Metabolism in Rats Pubmed:29495376
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD539Ra01 Recombinant Cytochrome P450 27B1 (CYP27B1) Positive Control; Immunogen; SDS-PAGE; WB.
PAD539Ra01 Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1) WB; IHC; ICC; IP.