Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat CYP27B1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Kidney Tissue
Image (II)
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Figure. Western Blot; Sample: Recombinant CYP27B1, Rat.
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Figure. Western Blot; Sample: Mouse Kidney Tissue.
Quality Guarantee
Instruction Manual
Certificate
Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1)
VDD1, PDDR; CYP1, P450c1; 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase; Cytochrome P450 Family 27 Subfamily B Polypeptide 1; Calcidiol 1-monooxygenase; VD3 1A hydroxylase
- Product No.PAD539Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPD539Ra01-Recombinant Cytochrome P450 27B1 (CYP27B1)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 114
US$ 266
US$ 380
US$ 950
US$ 3800
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CYP27B1. It has been selected for its ability to recognize CYP27B1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| Journal of Bone and Mineral Research | Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1α‐Hydroxylase Expression in Vascular Smooth Muscle Cells Pubmed:27074284 |
| Nutrients | Lead Affects Vitamin D Metabolism in Rats Pubmed:29495376 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD539Ra01 | Recombinant Cytochrome P450 27B1 (CYP27B1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD539Ra01 | Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1) | WB; IHC; ICC; IP. |