Previously we introducedthe common samples preparation methods including the preparation of blood(serum, plasma), tissue homogenates, cell lysates,etc.Nowwe’ll present preparation methods of uncommonsamples,such as sputum,bronchoalveolar lavage fluid, saliva, milk, hydrothorax and ascite, semen, etc.1.SputumWeightthe viscous part of sputum,mix the sputum with 0.1 % DTT (dithiothreitol) (two fold volume) and shake for 5 minutes at 37°C.Add PBS (twofold volume) andshake again for another 15-20 minutes. After Filtering through a 150 mesh wire net, remove particulates by centrifugation ofapproximately 1,000×gfor 10 minutes.Collect the supernatant and assay immediately or store samples ......2022-06-17

On 17 May 2022,Martin Schepelmann, School of Biosciences, Cardiff University, United Kingdom, and his team published a paper titled “Impaired Mineral Ion Metabolism in a Mouse Model of Targeted Calcium-Sensing Receptor (CaSR) Deletion from Vascular Smooth Muscle Cells” in Journal of the American Society of Nephrology, which suggested that the VSMC-CaSR directly contributes to total body mineral ion homeostasis.The kits [ELISA Kit for Klotho (KL), SEH757Mu] of Cloud-Clone brand was chosed in this article, we are so proud for supporting the reaserchers.Background: Impaired mineral ion metabolism is a hallmark of chronic kidney disease (CKD) -metabolic bone disorder. It can lead to pa......2022-06-15

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, which damages multiple tissues and organs, resulting from the production of autoantibodies to nuclear antigens. The clinical manifestations of SLE are highly heterogeneous, including cutaneous, musculoskeletal, renal, hematologic, neurologic, and other diverse symptoms.To date, nearly 180 genomic loci have been identifed as associated with SLE susceptibility in genetic studies in multiple ancestries. Recent genetic studies leverage diverse cell type–specifc epigenetic resources and other biological resources to draw better pictures of the disease pathogenesis, thus updating the genetic architecture of SLE. Such genet......2022-06-13

On May 13, 2022, Jens Walter, Department of Agricultural, Food and Nutritional Science, University of Alberta, Canada, and his team published a paper titled “Elucidating therole ofthegut microbiota inthephysiological effects ofdietary fiber” in Microbiome, which demonstrated the efficacy of purified dietary fibers when used as supplements and suggests that satietogeniceffects of arabinoxylan (AX) may be linked to bacterial taxa that ferment the fiber or utilize breakdown products. The kits [ELISA Kit for Lipopolysaccharide Binding Protein (LBP), SEB406Hu] of Cloud-Clone brand was chosed in this article, we are so proud for supporting the reaserchers.Background: Dietary fiber is an ......2022-06-09

Brain tumors are among the most devastating forms of cancerand glioblastoma (GBM) represents the most aggressive and lethalform of the disease. GBM is characterized by a poor prognosis, an extremely high mortality rate, and a high tendency for recurrence. Despite advances in surgery, radiation, and chemotherapy, the median survival time of GBM is only 15–16 months, and the 5-year overall survival rate is less than 5%. Thus, there is an urgent need to develop new effective therapeutic strategies for treating GBM. Recently, a number of studies have reported new advances in GBM research, which may help improve the clinical outcomes of GBM.1. M1-macrophage extracellular vesicles for effe......2022-05-27

On 21 March 2022, Jianli Ren, Department of Ultrasound and Chongqing Key Laboratory of Ultrasound Molecular Imaging, the Second Affiliated Hospital of Chongqing Medical University, P. R. China, and his team published a paper tittled “Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced immunochemotherapy” in Theranostics, which presented a nanocomposite that can restrict the growth of drug-resistant breast tumors and activates a strong immune response as well.The kits [ELISA Kit for Tumor Necrosis Factor Alpha (TNFa), SEA133Mu; ELISA Kit for Interferon Gamma (IFNg), SEA049Mu; ELISA Kit for Interleukin 6......2022-05-25