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Human A431 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human OCT4 Monoclonal Antibody (Catalog FAA424Hu02, red histogram) or Isotype control antibody (Catalog IS067-Mu02, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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Anti-Octamer Binding Transcription Factor 4 (OCT4) Monoclonal Antibody
POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3
- Product No.FAA424Hu02
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)C7
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
US$ 120
US$ 200
US$ 320
US$ 400
US$ 700
For more details, please contact local distributors!
USAGE
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Analytical Methods | A universal glucometer-based biosensor for portable and quantitative detection of transcription factors Rsc: Source |
| Oncology Letters | Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma Doi: 10.3892/ol.2018.9381 |
| Stem Cell Research | Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin |
| 博士论文 | Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products |
| Stem Cell Research | Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a … Pubmed: 31862609 |
| J Biochem Mol Toxicol | Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effect Pubmed:35187753 |