Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3
- Product No.MAA424Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C7
- Ig Isotype IgG2a Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA424Hu01-Recombinant Octamer Binding Transcription Factor 4 (OCT4)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.69mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 101
For more details, please contact local distributors! US$ 235 US$ 336 US$ 840 US$ 3360
SPECIFITY
The antibody is a mouse monoclonal antibody raised against OCT4. It has been selected for its ability to recognize OCT4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- Antibody Labeling Customized Service
- Protein A/G Purification Column
- Staining Solution for Cells and Tissue
- Positive Control for Antibody
- Tissue/Sections Customized Service
- Phosphorylated Antibody Customized Service
- Western Blot (WB) Experiment Service
- Immunohistochemistry (IHC) Experiment Service
- Immunocytochemistry (ICC) Experiment Service
- Flow Cytometry (FCM) Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Immunofluorescence (IF) Experiment Service
- Buffer
- DAB Chromogen Kit
- SABC Kit
- Long-arm Biotin Labeling Kit
- Real Time PCR Experimental Service
Magazine | Citations |
Analytical Methods | A universal glucometer-based biosensor for portable and quantitative detection of transcription factors Rsc: Source |
Oncology Letters | Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma Doi: 10.3892/ol.2018.9381 |
Stem Cell Research | Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin |
博士论文 | Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products |
Stem Cell Research | Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a … Pubmed: 31862609 |
J Biochem Mol Toxicol | Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effect Pubmed:35187753 |