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Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)

POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3

  • Product No.MAA424Hu23
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)2#
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 102  US$ 239  US$ 341  US$ 853  US$ 3410 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against OCT4. It has been selected for its ability to recognize OCT4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Analytical Methods A universal glucometer-based biosensor for portable and quantitative detection of transcription factors Rsc: Source
Oncology Letters Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma Doi: 10.3892/ol.2018.9381
Stem Cell Research Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin
博士论文 Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products
Stem Cell Research Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a … Pubmed: 31862609
J Biochem Mol Toxicol Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effect Pubmed:35187753
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA424Hu01 Recombinant Octamer Binding Transcription Factor 4 (OCT4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA424Hu01 Polyclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
MAA424Hu22 Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
MAA424Hu23 Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
MAA424Hu24 Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
MAA424Hu21 Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4) WB; IHC; ICC; IP.
FAA424Hu06 Anti-Octamer Binding Transcription Factor 4 (OCT4) Monoclonal Antibody Flow cytometry.
FAA424Hu04 Anti-Octamer Binding Transcription Factor 4 (OCT4) Monoclonal Antibody Flow cytometry.
FAA424Hu02 Anti-Octamer Binding Transcription Factor 4 (OCT4) Monoclonal Antibody Flow cytometry.
SEA424Hu ELISA Kit for Octamer Binding Transcription Factor 4 (OCT4) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA424Hu Multiplex Assay Kit for Octamer Binding Transcription Factor 4 (OCT4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.