Packages (Simulation)

Polyclonal Antibody to Cytokeratin 16 (CK16)

Image (I)

DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human KRT16 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Pancreatic cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Uterus Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human CK16 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Lung Tissue
Primary Ab: 30µg/ml Rabbit Anti-Human CK16 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Uterus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK16 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK16 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Figure. Western Blot; Sample: Recombinant CK16, Human.
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Tongue lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Skin lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Skin lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human KRT16 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cytokeratin-16-(CK16)-PAA516Hu01.pdf

Certificate

Polyclonal Antibody to Cytokeratin 16 (CK16)

KRT16; NEPPK; Keratin 16; Focal Non-Epidermolytic Palmoplantar Keratoderma; Keratin, type I cytoskeletal 16

Product No.PAA516Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA516Hu01-Recombinant Cytokeratin 16 (CK16)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 95 221 US$ 221 US$ 316 US$ 790 US$ 3160
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CK16. It has been selected for its ability to recognize CK16 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
Annals of Anatomy-Anatomischer Anzeiger	Normal microscopic anatomy of equine body and limb skin: A morphological and immunohistochemical study Pubmed:29730469
SLAS Discov	High-Throughput Screening to Identify Small Molecules That Selectively Inhibit APOL1 Protein Level in Podocytes 34218698

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPA516Hu01	Recombinant Cytokeratin 16 (CK16)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA516Hu01	Polyclonal Antibody to Cytokeratin 16 (CK16)	WB; IHC; ICC; IP.
LAA516Hu71	Biotin-Linked Polyclonal Antibody to Cytokeratin 16 (CK16)	WB; IHC; ICC.
LAA516Hu81	FITC-Linked Polyclonal Antibody to Cytokeratin 16 (CK16)	WB; IHC; ICC; IF.
MAA516Hu22	Monoclonal Antibody to Cytokeratin 16 (CK16)	WB; IHC; ICC; IP.
SEA516Hu	ELISA Kit for Cytokeratin 16 (CK16)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA516Hu	Multiplex Assay Kit for Cytokeratin 16 (CK16) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.