Packages (Simulation)

Polyclonal Antibody to Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)

Image (I)

DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure: FITC staining on IHC-P;
Sample: Hela cells.
AF488 staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Figure. Western Blot; Sample: Recombinant SHMT2, Human.
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Hela cell lysate; Lane4: Porcine Pancreas lysate
Primary Ab: 1µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: SHMT2 knockout 293T cell lysate;
Predicted MW: 54kd
Observed MW: 55kd
Primary Ab: 1µg/ml Rabbit Anti-Human SHMT2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human SHMT2 Polyclonal Antibody (Catalog PAE217Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

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Instruction Manual

Polyclonal-Antibody-to-Serine-Hydroxymethyltransferase-2--Mitochondrial-(SHMT2)-PAE217Hu01.pdf

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KO-Validated

Polyclonal Antibody to Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)

Serine methylase; Glycine hydroxymethyltransferase

Product No.PAE217Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE217Hu01-Recombinant Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreSus scrofa; Porcine (Pig)
ApplicationsWB,IHC,ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 105 245 US$ 245 US$ 350 US$ 875 US$ 3500
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SHMT2. It has been selected for its ability to recognize SHMT2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Flow cytometry:20µg/ml;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPE217Hu01	Recombinant Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAE217Hu01	Polyclonal Antibody to Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)	WB,IHC,ICC/IF; FCM